The retailer is to close its supermarket in Ince along with three others in England, with Morrisons instead looking to invest in four other stores.
A 45-day consultation process with staff has now begun to determine when the store on Makerfield Way will close and how to minimise job losses.
The Ince branch is the only store in the North West to close. The others are in Crawley in West Sussex, Shirley in Solihull, and Regents Circus in Swindon town centre.
Overall, the closures are set to put 400 jobs at risk.
A spokesperson for Morrisons said: “As we continue to focus on building a broader, stronger Morrisons we are constantly reviewing opportunities for new stores and the performance of our existing estate. We are planning to invest in four new stores this year and are today, regrettably, proposing to close our Ince store.
“We will now be going through a consultation process at the store and will be discussing how to reduce redundancies and, where possible, redeploy our highly valued colleagues within the business.”