St Modwen has let 8,000 sq ft of industrial space at Prescot Business Park to children's play operator SpaceWorld.
SpaceWorld has signed a 20-year stepped rental agreement at the 12-acre park.
Gill and Mark Baldwin, owners of SpaceWorld, said: "We have been eagerly waiting to start this venture up for some time, and can't wait to give parents something a little different to do with their children.
"We looked at a number of different locations, but couldn't find anything we felt could deliver our requirements, until we viewed Prescot Business Park. The overall presentation, proximity to local facilities and easy access to our customer base, all made it the perfect venue for the play centre.
"We opened mid-July and the facility is proving to be extremely popular."
Mike Hosie, St Modwen development manager, added: "The last two industrial units went under offer this week, which follows the recent achievement at Blackburn Innovation Centre which is now at 100% occupancy only nine months after its launch.
"SpaceWorld will certainly be one of our more entertaining tenants and we are pleased that alongside Knowsley MBC we have been able to provide the perfect facility for them to open their first venture. We wish them every success."
The new facility provides a play area for children and restaurant.
Prescot Business Park consists of 47,000 sq ft of industrial space and 21,000 sq ft of offices. The entire scheme is now 95% occupied.
The whole park is to be divided into three phases, with the first phase encompassing around 4 acres.
In addition to SpaceWorld, units have been taken up at Prescot Business Park by companies including flooring specialists Profile Interiors, Abacus Securities, CP Hood Electricals and Nicki Thomas Developments.
Prescot Business Park, close to Junction 2 of the M57, still has office suits available from 1,500 sq ft up to 6,000 sq ft.
CB Richard Ellis is the agent for the scheme.