The law firm has taken 17,200 sq ft across two floors in 55 Princess Street and plans to relocate its 70-strong Manchester team from Heron House in May.
The lease on the office is for ten years with a break at year five.
The move from Trowers' current Albert Square base to 55 Princess Street is part of expansion plans for the firm's Manchester team.
55 Princess Street is 55,000 sq ft, with 15,000 sq ft vacant.
OBI Property acted on behalf of the building landlord Benson Elliot. Trowers & Hamlins was represented by Knight Frank. JLL are joint leasing agents with OBI.
Mike Gaskell, managing partner of the Trowers & Hamlins Manchester office, said: "The firm has made – and continues to make – significant investments to grow our team in the North West, and as a result, we needed to find larger premises. We were impressed with the quality of the refurbishment and, in particular, the new reception and common areas of 55 Princess Street. The offices provide an ideal location and environment for our staff and clients, and we are very much looking forward to settling in."
Cube Real Estate asset manages the property on behalf of Benson Elliot.