Bolton-based Seddon Group has raised £330,000 for The Christie hospital in Manchester to help fund research for new cancer treatment known as the Magic Bullet.
The privately owned construction firm raised £80,000 more than its target figure and the final total is the culmination of a two year fundraising initiative instigated by Seddon in 2009.
Fundraising included donations from the company, a pound for pound sponsorship matching scheme for employees and fundraising throughout the group.
Seddon said colleagues, friends and family have spent the last two years organising and taking part in charity events such as quiz nights, sponsored slims, shark dive, car washes, dress down days and numerous sporting events which have covered a distance of over 7,500 miles.
The mileage was achieved by swimming 34 miles; rowing 40 miles; walking 1,814 miles, including the Yorkshire 3 peaks; cycling 4,915 miles; and running 706 miles, including two London Marathons completed by Jonathan Seddon, managing director of Seddon Construction.
Jonathan Seddon said: "We have been overwhelmed by the generosity and enthusiasm of our colleagues and are very proud to be able to hand over such a fantastic amount to The Christie. It is such a worthwhile cause and our donation will help research into the new Magic Bullet treatment which will provide a new way of treating cancer in the future.
"At some point in our lives many of us have or will come into contact with this awful disease. Our staff have been touched by what they've learnt about the work carried out at The Christie and this initiative has created a real buzz of excitement and camaraderie across the Seddon workforce."