Urban Splash has let the entire 20,000 sq ft Matchbox building in Garston to health club operator Fit Club.
The building, which was completed in December 2007 and has remained empty since, will be home to a new gym and will open later in the year.
Chris Metcalf, Fit Club's director, said: "The Matchbox is the perfect location for us and we're really pleased to have confirmed the deal. We were originally progressing with a 10,000 sq ft space in Matchworks but a lot of our suppliers kept asking why we weren't considering the Matchbox itself. With that in mind we viewed the space and knew we had to take it. We've got so much from this; a beautifully designed building, the established Matchworks community and a high profile location of the building."
The Matchbox was the first new build by Urban Splash in Liverpool and is located on the same site as its Matchworks redevelopment in south Liverpool.