Merseytravel's Mann Island headquarters will provide a base for the organiser of Liverpool's International Festival For Business.
The IFB Hub offices will be on the third and fourth floor of the building situated between the Port of Liverpool Building and the Albert Dock.
It will include a mayoral conference room where Liverpool mayor Cllr Joe Anderson will host a number of international meetings, as well as a media centre, exhibition space, meeting rooms and event space.
Businesses will be able to hire exhibition space at the Hub on what is described as the Trading Floor to market their services and products to visiting delegates.
The site was unveiled during a visit by cities and constitutions minister Greg Clark.
Clark said: "This building will be the heart of the IFB and will be a concentrated meeting and trading point for business people attending the festival from across the UK and abroad.
"The festival as a whole represents a once-in-a-generation opportunity for companies to make new global connections with potential partners and customers. The Hub will play a key role in this and will be a gathering point for delegates throughout the festival. It will also offer an opportunity for British businesses to boost their trade with organisations from across the world."
The International Festival for Business will take place between June and July 2014.