Eric Wright Special Projects has handed over the £1.7m development next to Burnley General Teaching Hospital, designed by DLA Freeman White Architects.
The 5,400 sq ft station replaces the former building on Trafalgar Street, and has been developed by One Partnership, a joint venture between Eric Wright Group and Community Health Partnerships.
The North West Ambulance Service has worked with East Lancashire Hospitals Trust on the scheme, which will meet demand for ambulances and emergencies across the Pennines and Lancashire.
As well as acting as an ambulance station, the building also houses welfare space and a training room.
The completion of the project opens up the former ambulance station located on Dent Row off Trafalgar Street for redevelopment; this will be transformed into a 90-space car park.
One Partnership managing director Christine Winstanley said: “The new building will help to meet the needs of the region’s health care system and will enable NWAS to deliver an improved service for its users.’’
“Our delivery partnership will continue to work together to improve healthcare services in the region as well as to forge wider redevelopment and economic opportunities for Burnley town centre.”