Liverpool auctioneer Sutton Kersh will include the Whitehouse pub on the corner of Berry Street and Duke Street in its next sale on 18 February.
The secretive Bristol-based graffiti artist was commissioned to create a piece in the Ropewalks area of the city for the 2004 Liverpool Biennial art festival. The giant rat holding a marker pen next to his red zig-zag doodles have been a prominent landmark ever since.
Sutton Kersh has set a guide price of £80,000 but the value only covers the building and does not include its worth as a work of art by one of the most famous contemporary artists in the world.
Whether the artwork is retained remains the decision of the purchaser. The panel of hoarding containing the Banksy signature, or tag, was stolen some years ago.
James Kersh, director of auctions at Sutton Kersh, said there has been strong pre-auction interest and the price may well exceed that of the guide.
- The sale is on Thursday 18 February at the Marriott Hotel in Queen Square, Liverpool city centre.